Journal:

Article Title: Irradiation up-regulates CD80 expression through two different mechanisms in spleen B cells, B lymphoma cells, and dendritic cells

doi: 10.1111/j.1365-2567.2004.01872.x

Figure Lengend Snippet: Irradiation selectively up-regulated CD80 expression in LPS-stimulated B cells. LPS-stimulated B cells were X-irradiated with 8Gy and incubated for 0–24 hr (a) or 12 hr (b) (a) Then, the cells were incubated with anti-CD80 mAb (16-10A1, hamster IgG), or hamster IgG as a control, and stained with biotinylated F(ab′)2 fraction of anti-hamster goat IgG antibody and FITC-conjugated streptoavidin. They were analysed on a flow cytometer. The results were shown as MFI. (b) The irradiated or nonirradiated cells were incubated with an antibody against CD80, CD86, MHC class II, IgM, CD54, or CD11a. They were stained with an appropriate biotinylated antibody and FITC–streptoavidin, and analysed on a flow cytometer.

Article Snippet: 16 Monoclonal antibodies (mAb), anti-I-A d E d (M5/114, rat immunoglobulin G (IgG) 2b 17 ), anti-mouse CD86 (GL-1, rat IgG 2a 18 ), anti-mouse CD4 (GK1.5, rat IgG 2b 19 ), anti-CD54 (intercellular adhesion molecule-1; ICAM-1) (YN1/1.7.4, rat IgG 2b 20 ), and anti-CD11a (leucocyte function-associated antigen-1; FD441.8, rat IgG2b 21 ), were obtained from the American Type Culture Collection (Rockville, MD), and were kindly made available by Dr H. Nariuchi from the Institute of Medical Science of the University of Tokyo.

Techniques: Irradiation, Expressing, Incubation, Control, Staining, Flow Cytometry